IMMUNOELECTRON MICROSCOPIC STUDY ON G CELLS AND D CELLS IN ANTRAL MUCOSA OF RAT / 解放军医学杂志

Revised immunoelectron microscopic technique was used to examine G cells and D cells in rat antral mucosa. The number of colloidal gold granules in G and D cells was analysed through quantitative analysis. The results showed that immunological granules of colloidal gold distributed in both G and D cells. Gastrin- or somatostatin-labeled granules were present mainly as lobation-like or island-like congeries. Most of the golden congeries dissociated in the cytoplasm of G or D cells, being in the basement membrane side. A few of the golden congeries located in nucleus. The number of golden granules was 107. 04 ?19. 68 and 83. 36?17. 58 per one G and D cell, respectively.

Study on the Action Mechanism of Insulin-Like Growth Factors and HBV-DNA Integration During Hepatocarcinogenesis / 中国医师杂志

Objective To observe the expressions of insulin-like growth factors(IGFs) and proliferating cell nuclear antigen(PCNA) in various kinds of liver tissues,we discussd the relationship among IGFs,PCNA and HBV-DNA integration in the process of hepatocarcinogenesis,provide some theoretical basis for the mechanism of hepatocarcinogenesis and clinical diagnosis and therapy of HCC.Methods 32 cases of HCC,32 cases of paracancerous liver tissues(PLT),12 cases of chronic active hepatitis(CAH),10 cases of fetal liver tissues(FLT) and 8 cases of normal adult liver tissues(NALT) were collected.Southern hybridization was used to detect HBV-DNA integration in various kinds of liver tissues.Then a colloid gold immunoelectron microscopic technique was used to make a systematic study on the expression and ultrastructural location of IGFs and PCNA in different liver tissues.Results The HBV-DNA integration was positively existed in 81 3%(26/32) of HCC and PLT,and 83 3%(10/12) in CAH,but the integration in NALT and FLT was no existed.PCNA was expressed by 87 5%(28/32) of HCC and 84 4%(27/32) of PLT,respectively.The postive rates of IGF-Ⅰ and IGF-ⅠR expressed by HCC were 40 6%(13/32) and 56 3%(18/32),those of IGF-Ⅰand IGF-ⅠR expressed by PLT were 50 0%(16/32) and 65 6%(21/32),respectively.The positive rates of IGF-Ⅱ and IGF-Ⅱ receptor(IGF-ⅡR) expressed by HCC were both 78 1%(25/32),and those expressed by PLT were both 81 3%(26/32).In the integration group of HBV-DNA had higher positive rates of IGF-Ⅱ and IGF-ⅡR,which were expressed by the HCC and PLT groups,than that of non-integration group of HBV-DNA(P